Supercritical Fluid Chromatography

Prochrom® Supersep systems are ideal for any chiral resolution, complex mixture purification or natural extract isolation. These provide solutions for greener, faster and cost-effective separations using the Supercritical Fluid chromatography (SFC) principle.


  • The use of supercritical CO2 decreases the amount of organic solvent required by 5 to 20 fold
  • Its unique CO2 recycling technology reduces CO2 consumption by up to 20 times, allowing you to drastically reduce both production costs and environmental impact
  • Scalable and fast solution
  • Stable, repeatable and robust process
  • Industrial programmable logic controller (PLC) equipped with PC interface with validated software based on GAMP 5 and compliant with 21 CFR part 11

SFC offering includes

1. Prochrom® SFC columns

Fully automated Hipersep HPLC systems are designed for process development, pilot-scale and production-scale chromatographic separations.


  • Preparative chromatography columns adapted to the high pressure requirement of SFC and based on Prochrom® DAC (Dynamic Axial Compression) technology
  • The DAC system consists of a piston which maintains a dynamic axial compression on the stationary phase. This provides packing with an optimum bed density and stability ensuring an efficient separation, easy and direct scale-up and a consistent performance at any scale of production and over extended periods of time
Column Diameter (mm) 50 80 100 150 200
Max. Bed Length (mm) 350 450
Pressure Rating (bar) 300

2. Prochrom® Supersep chromatography systems


  • Fully automated Supersep™ SFC systems are designed for process development and pilot-scale chromatographic separations
  • A robust and efficient design
  • Optimal industrial design allowing for 24/7 continuous operation
  • High productivity and yield resulting from excellent process reproducibility
  • Reliability of instrumentation and easy access for reduced maintenance
  • Compatibility with separation methods developed on any analytical SFC system
  • A cost-effective and environmentally friendly solution
  • Flexible CO2 supply (gas or liquid) to minimize CAPEX and energy needs
  • Patented CO2 recycling device to reduce CO2 consumption by 10 to 20 times
  • Contained system for feed and fraction collection to prevent any toxic aerosols
  • High-pressure cyclones for high recovery yields
  • Proprietary automatic cleaning-in-place feature for faster product changeovers
  • All SFC systems can be operated under cGMP conditions. They are fully qualified and extensively tested during factory acceptance testing (FAT) and site acceptance testing (SAT)