Description
Applications
- Pre-purification and intermediate purification of insulin
- Vaccines and viruses
- Proteins such as monoclonal antibodies and blood/plasma fractions
LPLC offerings include
The range, very complete with lab- to large-scale equipment, includes:
- Prochrom®-Bio Columns
- Packing & Unpacking Stations
- Prochrom® Hipersep-Bio Chromatography Systems
1. Prochrom®-Bio Columns
Prochrom®-Bio columns are the result of Novasep’s experience in developing, supplying and operating cGMP processes using preparative and process-scale high performance and low pressure liquid chromatography columns. Prochrom®-Bio columns’ unique design includes a hydraulically actuated piston and specially designed packing/unpacking valves to provide consistent and optimum performance.
Features
- Standard column diameters range from 150 to 1,400 mm
- Material of construction: acrylic or stainless steel
- 5 bar operating pressure
- Easy and fast maintenance
- DESP or ASME code of construction
- Sanitary design
- Operator safety: contained packing & unpacking operations, column design, etc
- Easy and fast packing and unpacking
- Optimum and reproducible performance
- Guaranteed scale-up
- Versatility: excellent and proven performance with a wide variety of resins
2. Packing and Unpacking Systems
Novasep offers 3 models of packing and unpacking stations adapted to the column size. These stations provide:
- Highest efficiency for any kind of resin thanks to the specially designed packing & unpacking valves and the hydraulically actuated piston
- Reduced downtime with fast and easy packing and unpacking operations
- Minimized repacking time of recycled resin which needs only a small amount of buffer for unpacking (2 to 3 CVs)
- High level of reproducibility due to automated functions
- Pre-programmed or easily configurable packing & unpacking methods
| Column Name | Column Diameter (mm) | Maximum BedVolume (L) | Packing & Unpacking Station | Prochrom® Hipersep-Bio System | Flow-Rate Range (LHP) |
|---|---|---|---|---|---|
| LP150.500.VE 5 | 150 | 8 | PU S | Pilot | 1- 50 |
| LP200.500.VE 5 | 200 | 12 | PU S | S | 1- 150 |
| LP300.500.VE 5 | 300 | 32 | PU S | S | 1- 150 |
| LP450.500.VE 5 | 450 | 70 | PU S / M | M | 20-500 |
| LP600.500.VE 5 | 600 | 125 | PU M | M | 20-500 |
| LP800.350.VE 5 | 800 | 150 | PU M / L | L | 50 – 1200 |
| LP1000.350.VE 5 | 1000 | 230 | PU L | L | 50 – 1200 |
| LP1200.350.VE 5 | 1200 | 330 | PU L | XL | 200 – 3000 |
| LP1400.350.VE 5 | 1400 | 450 | PU L | XL | 200 – 3000 |
3. Prochrom® Hipersep-Bio Chromatography Systems
Novasep’s fully automated Prochrom® Hipersep-Bio LPLC systems are designed for process development, pilot-scale and production-scale downstream processing. Scalability is ensured using the same design from lab to large industrial units. Prochrom® Hipersep-Bio systems suit any biochromatography columns and offer maximum performance with Prochrom®-Bio columns for bed volumes from 1 to 450 L and column diameters from 150 mm to 1,400 mm I.D.
Features
- Reduced investment with an exceptional turn-down ratio due to the optimal design and choice of components. Very accurate in-line dilution control based on volume or conductivity means reduced tank and space requirements
- High productivity and yield resulting from high accuracy and stability of operating parameters (velocities, buffer composition, temperature, etc.) as well as excellent process reproducibility.
- Reliability of instrumentation and “open design” (providing easy access to parts) means reduced maintenance and, thus, reduced operating costs
- Validated and user-friendly software for easier start-up, training and on-site qualifications (see section below)
| Prochrom® Hipersep-Bio Systems | |||||
|---|---|---|---|---|---|
| Standard Features | Pilot | S | M | L | XL |
| Flow Rate Range(LPH) | 1-50 | 1-150 | 20-500 | 50-1200 | 200-3000 |
| Pressure Rating(bar) | 5 | ||||
| Buffer Preparation | High Performance, PAT-Compliant in-line dilution | ||||
| Fraction Collection | 5 to 10 Valves, multiple automated fraction collection modes | ||||
| Detection | Air,pressure, pH, conductivity, UV | ||||
| Control System | Siemens WinAC platform-iFix SCADA | ||||
